An ultrasensitive rapid immunocytotoxicity assay for detecting Clostridium difficile toxins.
نویسندگان
چکیده
We describe a novel ultrasensitive cell-based immunocytotoxicity assay for detecting Clostridium difficile toxin A and B. The assay is simple to perform with a turnaround time of approximately 3 h . It is particularly sensitive in detecting TcdA at a level less then 1 pg/ml. Using this assay, we were able to detect the presence of C. difficile toxins in the fecal and serum specimens of experimentally infected piglets.
منابع مشابه
Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs
The aim of this study was to isolate Clostridium difficile from dogs’ faeces, and to study the frequency of its virulence genes. A total of 151 samples of dogs’ faeces were collected. The isolation of C. difficile was performed by using the bacterial culture methods followed by DNA extraction using boiling method. Multiplex PCR method was performed for identification of tcdA, tcdB, cdtA and cdt...
متن کاملIdentification of Toxemia in Patients with Clostridium difficile Infection
Toxemia can develop in Clostridium difficile-infected animals, and correlates with severe and fulminant disease outcomes. Circumstantial evidence suggests that toxemia may occur in patients with C. difficile infection (CDI), but positive diagnosis is extremely rare. We analyzed the potential for C. difficile toxemia in patients, determined its characteristics, and assessed challenges. C. diffic...
متن کاملExtraction and Sensitive Detection of Toxins A and B from the Human Pathogen Clostridium difficile in 40 Seconds Using Microwave-Accelerated Metal-Enhanced Fluorescence
Clostridium difficile is the primary cause of antibiotic associated diarrhea in humans and is a significant cause of morbidity and mortality. Thus the rapid and accurate identification of this pathogen in clinical samples, such as feces, is a key step in reducing the devastating impact of this disease. The bacterium produces two toxins, A and B, which are thought to be responsible for the major...
متن کاملThe effect of clostridium difficile Toxins Aand B on ligated rabbit IIeal loop and cultured cell link BK
clostridium difficile has been recognized as the major cause of pseudomembranous colitis.this bacterium produces two toxins(an enterotoxin -cytotoxin and a potent cytotoxin called toxin A and toxin B erespectively).these toxins have implicated in pathogenesis of the disease.however,histopathological effects of their molecular mass less than 100KDa have been essayed.in the persent study,we exami...
متن کاملSystemic dissemination of Clostridium difficile toxins A and B is associated with severe, fatal disease in animal models.
BACKGROUND Clostridium difficile infection (CDI) can cause a wide range of disease, from mild diarrhea to fulminant systemic disease. The incidence of systemic CDI with fatal consequence has increased rapidly in recent years. METHODS Using an ultrasensitive cytotoxicity assay, we measured C. difficile toxin A (TcdA) and C. difficile toxin B (TcdB) in sera and body fluids of piglets and mice e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of microbiological methods
دوره 78 1 شماره
صفحات -
تاریخ انتشار 2009